Defining the boundaries and characterizing the landscape of functional genome expression in vascular tissues of Populus using shotgun proteomics.
Identifieur interne : 002B40 ( Main/Exploration ); précédent : 002B39; suivant : 002B41Defining the boundaries and characterizing the landscape of functional genome expression in vascular tissues of Populus using shotgun proteomics.
Auteurs : Paul Abraham [États-Unis] ; Rachel Adams ; Richard J. Giannone ; Udaya Kalluri ; Priya Ranjan ; Brian Erickson ; Manesh Shah ; Gerald A. Tuskan ; Robert L. HettichSource :
- Journal of proteome research [ 1535-3907 ] ; 2012.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (MeSH), Biologie informatique (MeSH), Cartographie peptidique (MeSH), Données de séquences moléculaires (MeSH), Expression des gènes (MeSH), Faisceau vasculaire des plantes (génétique), Faisceau vasculaire des plantes (métabolisme), Fragments peptidiques (composition chimique), Génome végétal (MeSH), Paroi cellulaire (métabolisme), Polymorphisme de nucléotide simple (MeSH), Populus (génétique), Populus (métabolisme), Protéines végétales (composition chimique), Protéines végétales (génétique), Protéines végétales (métabolisme), Protéolyse (MeSH), Protéome (composition chimique), Protéome (génétique), Protéome (métabolisme), Protéomique (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Spectrométrie de masse en tandem (MeSH), Stress physiologique (MeSH), Séquence d'acides aminés (MeSH).
- MESH :
- composition chimique : Fragments peptidiques, Protéines végétales, Protéome.
- génétique : Faisceau vasculaire des plantes, Populus, Protéines végétales, Protéome.
- métabolisme : Faisceau vasculaire des plantes, Paroi cellulaire, Populus, Protéines végétales, Protéome.
- Analyse de séquence d'ADN, Biologie informatique, Cartographie peptidique, Données de séquences moléculaires, Expression des gènes, Génome végétal, Polymorphisme de nucléotide simple, Protéolyse, Protéomique, Régulation de l'expression des gènes végétaux, Spectrométrie de masse en tandem, Stress physiologique, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Cell Wall (metabolism), Computational Biology (MeSH), Gene Expression (MeSH), Gene Expression Regulation, Plant (MeSH), Genome, Plant (MeSH), Molecular Sequence Data (MeSH), Peptide Fragments (chemistry), Peptide Mapping (MeSH), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Vascular Bundle (genetics), Plant Vascular Bundle (metabolism), Polymorphism, Single Nucleotide (MeSH), Populus (genetics), Populus (metabolism), Proteolysis (MeSH), Proteome (chemistry), Proteome (genetics), Proteome (metabolism), Proteomics (MeSH), Sequence Analysis, DNA (MeSH), Stress, Physiological (MeSH), Tandem Mass Spectrometry (MeSH).
- MESH :
- chemical , chemistry : Peptide Fragments, Plant Proteins, Proteome.
- chemical , genetics : Plant Proteins, Proteome.
- genetics : Plant Vascular Bundle, Populus.
- metabolism : Cell Wall, Plant Proteins, Plant Vascular Bundle, Populus, Proteome.
- Amino Acid Sequence, Computational Biology, Gene Expression, Gene Expression Regulation, Plant, Genome, Plant, Molecular Sequence Data, Peptide Mapping, Polymorphism, Single Nucleotide, Proteolysis, Proteomics, Sequence Analysis, DNA, Stress, Physiological, Tandem Mass Spectrometry.
Abstract
Current state-of-the-art experimental and computational proteomic approaches were integrated to obtain a comprehensive protein profile of Populus vascular tissue. This featured: (1) a large sample set consisting of two genotypes grown under normal and tension stress conditions, (2) bioinformatics clustering to effectively handle gene duplication, and (3) an informatics approach to track and identify single amino acid polymorphisms (SAAPs). By applying a clustering algorithm to the Populus database, the number of protein entries decreased from 64,689 proteins to a total of 43,069 protein groups, thereby reducing 7505 identified proteins to a total of 4226 protein groups, in which 2016 were singletons. This reduction implies that ∼50% of the measured proteins shared extensive sequence homology. Using conservative search criteria, we were able to identify 1354 peptides containing a SAAP and 201 peptides that become tryptic due to a K or R substitution. These newly identified peptides correspond to 502 proteins, including 97 previously unidentified proteins. In total, the integration of deep proteome measurements on an extensive sample set with protein clustering and peptide sequence variants provided an exceptional level of proteome characterization for Populus, allowing us to spatially resolve the vascular tissue proteome.
DOI: 10.1021/pr200851y
PubMed: 22003893
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Defining the boundaries and characterizing the landscape of functional genome expression in vascular tissues of Populus using shotgun proteomics.</title><author><name sortKey="Abraham, Paul" sort="Abraham, Paul" uniqKey="Abraham P" first="Paul" last="Abraham">Paul Abraham</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831</wicri:regionArea><wicri:noRegion>Tennessee 37831</wicri:noRegion></affiliation></author><author><name sortKey="Adams, Rachel" sort="Adams, Rachel" uniqKey="Adams R" first="Rachel" last="Adams">Rachel Adams</name></author><author><name sortKey="Giannone, Richard J" sort="Giannone, Richard J" uniqKey="Giannone R" first="Richard J" last="Giannone">Richard J. Giannone</name></author><author><name sortKey="Kalluri, Udaya" sort="Kalluri, Udaya" uniqKey="Kalluri U" first="Udaya" last="Kalluri">Udaya Kalluri</name></author><author><name sortKey="Ranjan, Priya" sort="Ranjan, Priya" uniqKey="Ranjan P" first="Priya" last="Ranjan">Priya Ranjan</name></author><author><name sortKey="Erickson, Brian" sort="Erickson, Brian" uniqKey="Erickson B" first="Brian" last="Erickson">Brian Erickson</name></author><author><name sortKey="Shah, Manesh" sort="Shah, Manesh" uniqKey="Shah M" first="Manesh" last="Shah">Manesh Shah</name></author><author><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name></author><author><name sortKey="Hettich, Robert L" sort="Hettich, Robert L" uniqKey="Hettich R" first="Robert L" last="Hettich">Robert L. Hettich</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22003893</idno><idno type="pmid">22003893</idno><idno type="doi">10.1021/pr200851y</idno><idno type="wicri:Area/Main/Corpus">002C57</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002C57</idno><idno type="wicri:Area/Main/Curation">002C57</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002C57</idno><idno type="wicri:Area/Main/Exploration">002C57</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Defining the boundaries and characterizing the landscape of functional genome expression in vascular tissues of Populus using shotgun proteomics.</title><author><name sortKey="Abraham, Paul" sort="Abraham, Paul" uniqKey="Abraham P" first="Paul" last="Abraham">Paul Abraham</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831</wicri:regionArea><wicri:noRegion>Tennessee 37831</wicri:noRegion></affiliation></author><author><name sortKey="Adams, Rachel" sort="Adams, Rachel" uniqKey="Adams R" first="Rachel" last="Adams">Rachel Adams</name></author><author><name sortKey="Giannone, Richard J" sort="Giannone, Richard J" uniqKey="Giannone R" first="Richard J" last="Giannone">Richard J. Giannone</name></author><author><name sortKey="Kalluri, Udaya" sort="Kalluri, Udaya" uniqKey="Kalluri U" first="Udaya" last="Kalluri">Udaya Kalluri</name></author><author><name sortKey="Ranjan, Priya" sort="Ranjan, Priya" uniqKey="Ranjan P" first="Priya" last="Ranjan">Priya Ranjan</name></author><author><name sortKey="Erickson, Brian" sort="Erickson, Brian" uniqKey="Erickson B" first="Brian" last="Erickson">Brian Erickson</name></author><author><name sortKey="Shah, Manesh" sort="Shah, Manesh" uniqKey="Shah M" first="Manesh" last="Shah">Manesh Shah</name></author><author><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name></author><author><name sortKey="Hettich, Robert L" sort="Hettich, Robert L" uniqKey="Hettich R" first="Robert L" last="Hettich">Robert L. Hettich</name></author></analytic><series><title level="j">Journal of proteome research</title><idno type="eISSN">1535-3907</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Cell Wall (metabolism)</term><term>Computational Biology (MeSH)</term><term>Gene Expression (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genome, Plant (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Peptide Fragments (chemistry)</term><term>Peptide Mapping (MeSH)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plant Vascular Bundle (genetics)</term><term>Plant Vascular Bundle (metabolism)</term><term>Polymorphism, Single Nucleotide (MeSH)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Proteolysis (MeSH)</term><term>Proteome (chemistry)</term><term>Proteome (genetics)</term><term>Proteome (metabolism)</term><term>Proteomics (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Stress, Physiological (MeSH)</term><term>Tandem Mass Spectrometry (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN (MeSH)</term><term>Biologie informatique (MeSH)</term><term>Cartographie peptidique (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Expression des gènes (MeSH)</term><term>Faisceau vasculaire des plantes (génétique)</term><term>Faisceau vasculaire des plantes (métabolisme)</term><term>Fragments peptidiques (composition chimique)</term><term>Génome végétal (MeSH)</term><term>Paroi cellulaire (métabolisme)</term><term>Polymorphisme de nucléotide simple (MeSH)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Protéolyse (MeSH)</term><term>Protéome (composition chimique)</term><term>Protéome (génétique)</term><term>Protéome (métabolisme)</term><term>Protéomique (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Spectrométrie de masse en tandem (MeSH)</term><term>Stress physiologique (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Peptide Fragments</term><term>Plant Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Fragments peptidiques</term><term>Protéines végétales</term><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Vascular Bundle</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Faisceau vasculaire des plantes</term><term>Populus</term><term>Protéines végétales</term><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Plant Proteins</term><term>Plant Vascular Bundle</term><term>Populus</term><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Faisceau vasculaire des plantes</term><term>Paroi cellulaire</term><term>Populus</term><term>Protéines végétales</term><term>Protéome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Computational Biology</term><term>Gene Expression</term><term>Gene Expression Regulation, Plant</term><term>Genome, Plant</term><term>Molecular Sequence Data</term><term>Peptide Mapping</term><term>Polymorphism, Single Nucleotide</term><term>Proteolysis</term><term>Proteomics</term><term>Sequence Analysis, DNA</term><term>Stress, Physiological</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Biologie informatique</term><term>Cartographie peptidique</term><term>Données de séquences moléculaires</term><term>Expression des gènes</term><term>Génome végétal</term><term>Polymorphisme de nucléotide simple</term><term>Protéolyse</term><term>Protéomique</term><term>Régulation de l'expression des gènes végétaux</term><term>Spectrométrie de masse en tandem</term><term>Stress physiologique</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Current state-of-the-art experimental and computational proteomic approaches were integrated to obtain a comprehensive protein profile of Populus vascular tissue. This featured: (1) a large sample set consisting of two genotypes grown under normal and tension stress conditions, (2) bioinformatics clustering to effectively handle gene duplication, and (3) an informatics approach to track and identify single amino acid polymorphisms (SAAPs). By applying a clustering algorithm to the Populus database, the number of protein entries decreased from 64,689 proteins to a total of 43,069 protein groups, thereby reducing 7505 identified proteins to a total of 4226 protein groups, in which 2016 were singletons. This reduction implies that ∼50% of the measured proteins shared extensive sequence homology. Using conservative search criteria, we were able to identify 1354 peptides containing a SAAP and 201 peptides that become tryptic due to a K or R substitution. These newly identified peptides correspond to 502 proteins, including 97 previously unidentified proteins. In total, the integration of deep proteome measurements on an extensive sample set with protein clustering and peptide sequence variants provided an exceptional level of proteome characterization for Populus, allowing us to spatially resolve the vascular tissue proteome.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22003893</PMID><DateCompleted><Year>2012</Year><Month>05</Month><Day>01</Day></DateCompleted><DateRevised><Year>2012</Year><Month>01</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1535-3907</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><Issue>1</Issue><PubDate><Year>2012</Year><Month>Jan</Month><Day>01</Day></PubDate></JournalIssue><Title>Journal of proteome research</Title><ISOAbbreviation>J Proteome Res</ISOAbbreviation></Journal><ArticleTitle>Defining the boundaries and characterizing the landscape of functional genome expression in vascular tissues of Populus using shotgun proteomics.</ArticleTitle><Pagination><MedlinePgn>449-60</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/pr200851y</ELocationID><Abstract><AbstractText>Current state-of-the-art experimental and computational proteomic approaches were integrated to obtain a comprehensive protein profile of Populus vascular tissue. This featured: (1) a large sample set consisting of two genotypes grown under normal and tension stress conditions, (2) bioinformatics clustering to effectively handle gene duplication, and (3) an informatics approach to track and identify single amino acid polymorphisms (SAAPs). By applying a clustering algorithm to the Populus database, the number of protein entries decreased from 64,689 proteins to a total of 43,069 protein groups, thereby reducing 7505 identified proteins to a total of 4226 protein groups, in which 2016 were singletons. This reduction implies that ∼50% of the measured proteins shared extensive sequence homology. Using conservative search criteria, we were able to identify 1354 peptides containing a SAAP and 201 peptides that become tryptic due to a K or R substitution. These newly identified peptides correspond to 502 proteins, including 97 previously unidentified proteins. In total, the integration of deep proteome measurements on an extensive sample set with protein clustering and peptide sequence variants provided an exceptional level of proteome characterization for Populus, allowing us to spatially resolve the vascular tissue proteome.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Abraham</LastName><ForeName>Paul</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Adams</LastName><ForeName>Rachel</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Giannone</LastName><ForeName>Richard J</ForeName><Initials>RJ</Initials></Author><Author ValidYN="Y"><LastName>Kalluri</LastName><ForeName>Udaya</ForeName><Initials>U</Initials></Author><Author ValidYN="Y"><LastName>Ranjan</LastName><ForeName>Priya</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Erickson</LastName><ForeName>Brian</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Shah</LastName><ForeName>Manesh</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Tuskan</LastName><ForeName>Gerald A</ForeName><Initials>GA</Initials></Author><Author ValidYN="Y"><LastName>Hettich</LastName><ForeName>Robert L</ForeName><Initials>RL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>11</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Proteome Res</MedlineTA><NlmUniqueID>101128775</NlmUniqueID><ISSNLinking>1535-3893</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010446">Peptide Fragments</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019295" MajorTopicYN="N">Computational Biology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018745" MajorTopicYN="Y">Genome, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010446" MajorTopicYN="N">Peptide Fragments</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010449" MajorTopicYN="N">Peptide Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058526" MajorTopicYN="N">Plant Vascular Bundle</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020641" MajorTopicYN="N">Polymorphism, Single Nucleotide</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059748" MajorTopicYN="N">Proteolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053719" MajorTopicYN="N">Tandem Mass Spectrometry</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>10</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>10</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>5</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22003893</ArticleId><ArticleId IdType="doi">10.1021/pr200851y</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Adams, Rachel" sort="Adams, Rachel" uniqKey="Adams R" first="Rachel" last="Adams">Rachel Adams</name><name sortKey="Erickson, Brian" sort="Erickson, Brian" uniqKey="Erickson B" first="Brian" last="Erickson">Brian Erickson</name><name sortKey="Giannone, Richard J" sort="Giannone, Richard J" uniqKey="Giannone R" first="Richard J" last="Giannone">Richard J. Giannone</name><name sortKey="Hettich, Robert L" sort="Hettich, Robert L" uniqKey="Hettich R" first="Robert L" last="Hettich">Robert L. Hettich</name><name sortKey="Kalluri, Udaya" sort="Kalluri, Udaya" uniqKey="Kalluri U" first="Udaya" last="Kalluri">Udaya Kalluri</name><name sortKey="Ranjan, Priya" sort="Ranjan, Priya" uniqKey="Ranjan P" first="Priya" last="Ranjan">Priya Ranjan</name><name sortKey="Shah, Manesh" sort="Shah, Manesh" uniqKey="Shah M" first="Manesh" last="Shah">Manesh Shah</name><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Abraham, Paul" sort="Abraham, Paul" uniqKey="Abraham P" first="Paul" last="Abraham">Paul Abraham</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002B40 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002B40 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:22003893
|texte= Defining the boundaries and characterizing the landscape of functional genome expression in vascular tissues of Populus using shotgun proteomics.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22003893" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |